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    21st International Ayurveda Symposium

    September, 13 - 15, 2019 in Birstein, Germany



In vitro Antimicrobial activity of Guduchi (Tinospora cordifolia) Satva

Prof. Brahmananda Mahapatra (LV)

Introduction: Guduchi Satva (GS), the sediment starchy aqueous extract obtained from Guduchi (Tinospora cordifolia) has been reported as highly nutritive and digestive and used in many diseases for preventive as well as curative. The present study was designed to evaluate and compare the antimicrobial activity of GS, prepared from the stem climbs over the neem (Azadirachta indica) tree and that climbs over the other trees.
Methods: Two test samples of GS were prepared following classical guidelines using Guduchi collected from neem (Azadirachta indica) tree as GS-l and Guduchi collected from other than neem tree as GS-ll. Recommended microbial strains – such as Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger were used for anti- microbial evaluation. Standards used in this study were Ciprofloxacin and Nystatin against bacteria and fungi respectively. Antimicrobial study was done by Disc Diffusion Method.
Results: The drug concentration of crude extract for test solution was - 25%, 50%, 75% and 100%. Both the two test samples (GS-l and GS-ll) exhibited remarkable antibacterial and antifungal activity. Statistical study was not done as the study is preliminary screening for antimicrobial activity. The results were proposed based on the difference in zone of inhibition (ZOI) for the tested samples. But when the concentration was increased the activity was significant. Maximum ZOI for antibacterial was against P. aeruginosa and S.pyogenes while in antifungal it was against C. albicans.
Conclusion: Both the test samples of GS showed effective antimicrobial effect and more significant in higher concentration. This can be further evaluated on various microorganisms, clinical trials and made available in the form of external ointments for wound, ulcer and burn etc.
Keywords: Antimicrobial activity, Guduchi, Satva, Tinospora cordifolia.

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